Wednesday, April 9, 2008

 

Wednes-Day 2

Bio- HW: Outline Chapter 13.1 due Thursday;
Outlines 13.2 and 13.3 are due on Monday before the exam.
We discussed the purpose and mechanism of RECOMBINANT DNA.This process involves the use of RESTRICTION ENZYMES to cut two DNA molecules (usually a bacterial chromosome/plasmid and a eukaryotic chromosome) at specific sites/sequences of nucleotides. The cutting of the double stranded DNA leaves identical "sticky ends" to two DNA segments, which then hydrogen-bond/join together a.k.a "recombine" to form a single chromosome containing genes from both the plasmid and the eukaryotic chromosome. The recombinant DNA plasmid is then re-inserted, via some vector/virus, into another bacterium, which then replicates asexually and produces identical copies of the recombinant DNA plasmid. This technology can be used to insert virtually any human gene into a bacterium so that the bacteria can produce the human protein/enzyme that is coded for by that gene. This technology has revolutionized biology and medicine.

Chem 7/8- we took the multiple choice part of the exam and then we began our new unit on Solutions.
Chem 9- we took the multiple choice part of the exam.




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